文献简介

出版社:Department of Dermatology

作  者:Mathias Sulk, Stephan Seeliger, Jerome Aubert

编  号:10.1038/jid.2011.424

关键字:rosacea | distribution | expression | TRPV

年  份:2012   点击量:634

文献摘要

Rosacea is a frequent chronic inflammatory skin disease of unknown etiology. Because early rosacea reveals all characteristics of neurogenic inflammation, a central role of sensory nerves in its pathophysiology has been discussed. Neuroinflammatory mediators and their receptors involved in rosacea are poorly defined. Good candidates may be transient receptor potential (TRP) ion channels of vanilloid type (TRPV), which can be activated by many trigger factors of rosacea. Interestingly, TRPV2, TRPV3, and TRPV4 are expressed by both neuronal and non-neuronal cells. Here, we analyzed the expression and distribution of TRPV receptors in the various subtypes of rosacea on non-neuronal cells using immunohistochemistry, morphometry, double immunoflourescence, and quantitative real-time PCR (qRT-PCR) as compared with healthy skin and lupus erythematosus. Our results show that dermal immunolabeling of TRPV2 and TRPV3 and gene expression of TRPV1 is significantly increased in erythematotelangiectatic rosacea (ETR). Papulopustular rosacea (PPR) displayed an enhanced immunoreactivity for TRPV2, TRPV4, and also of TRPV2 gene expression. In phymatous rosacea (PhR)-affected skin, dermal immunostaining of TRPV3 and TRPV4 and gene expression of TRPV1 and TRPV3 was enhanced, whereas epidermal TRPV2 staining was decreased. Thus, dysregulation of TRPV channels also expressed by non-neuronal cells may be critically involved in the initiation and/or development of rosacea. TRP ion channels may be targets for the treatment of rosacea.

酒渣鼻是一种病因不明的常见慢性炎症性皮肤病。由于早期酒渣鼻揭示了神经性炎症的所有特征,因此,在它的病理生理学中,感觉神经的重要作用已被探讨。酒渣鼻所涉及的神经炎症介质和它们的受体很难确定。好的备选可能是香草酸亚型瞬时受体电位(TRP)离子通道(TRPV),它可通过许多酒渣鼻的触发因素进行激活。有趣的是,TRPV2TRPV3TRPV4都是通过神经元和非神经元细胞进行表达。这里,与健康的皮肤和红斑狼疮相比,我们在非神经元细胞上通过使用免疫组织化学、形态计量法、免疫荧光双标和实时定量PCR技术(qRT-PCR)分析了不同亚型的酒渣鼻中TRPV受体的表达和分布。我们的研究结果表明在红斑毛细血管扩张型酒渣鼻(ETR)中TRPV2TRPV3真皮免疫标记和TRPV1的基因表达显著增加。丘疹脓疱型酒糟鼻(PPR)揭示了TRPV2TRPV4的免疫反应增强以及TRPV2基因表达增加。在肿块型酒糟鼻(PHR)受累的皮肤中,TRPV3TRPV4的真皮免疫染色增强,TRPV1TRPV3的基因表达增强,而表皮的TRPV2染色下降。因此,TRPV通道的失调也通过非神经元细胞进行表达,并且可能与酒渣鼻的发病和/或发展紧密相关。治疗酒渣鼻可能应该把TRP离子通道作为目标对象。