文献简介

出版社:Trop Anim Health Prod (2012) 44:689–692

作  者:Tania Alen Coutinho , Andrea Micke Moreno

编  号:10.1007/s11250-011-9980-4

关键字:Erysipelothrix rhusiopathiae . ,ayassu pecari ,MIC .,PFGE , Serotyping

年  份:2012   点击量:606

文献摘要

  Erysipelothrix rhusiopathiae causes a variety of diseases in many animal species, including human beings. Most human infections caused by this pathogen are related to occupational exposure, and swine are considered to be the most important reservoir of E. rhusiopathiae. The white lipped peccary (Tayassu pecari) is an ungulate that has some genetic relationship to swine, and since the demand for T. pecari meat has recently increased in Brazil and nothing is known about the relationship of this peccary with the occupational zoonotic agent, E. rhusiopathiae, an investigation on the matter was conducted. Tonsils from 21 T. pecari slaughtered in southern Brazil were examined, and one animal was positive for E. rhusiopathiae isolation. Five colonies of this positive specimen had their species identity confirmed by PCR, and were characterized by serotyping, broth microdilution susceptibility test, and pulsed field gel electrophoresis (PFGE). All colonies belonged to serotype 2b, and presented identical susceptibility profiles. Nevertheless, the five colonies showed three different PFGE profiles, demonstrating the occurrence of infection by different E. rhusiopathiae genotypes. This is the first report of E. rhusiopathiae infection in T. pecari as well as the first description of animals carrying different E. Rhusiopathiae genotypes.

  猪红斑丹毒丝菌在许多动物包括人类身上引起各种各样的疾病。大多数人类感染都与职业性的接触这种病原体有关,并且猪被认为是猪丹毒丝菌中最主要的宿主。。白唇野猪(白唇西貒)是一种与猪有亲缘关系的有蹄类动物,由于最近在巴西T. pecari肉的需求增加,并且我们对这种野猪与猪丹毒丝菌这一人畜共患病的关系一无所知,因此我们对此事进行了研究。对在巴西南部屠杀的21只白唇西貒的扁桃体进行检查,并在其中的一只身上分离出了阳性的猪丹毒丝菌。通过PCR对阳性标本中的五菌落进行物种鉴定,并进行血清分型、肉汤稀释法药敏试验、脉冲场凝胶电泳(PFGE)对其特征进行鉴别。所有的菌落都属于2b血清型,并显示了相同的敏感带。然而,五个菌落显示出三个不同的脉冲场凝胶电泳(PFGE)带,证明了不同猪丹毒丝菌基因型感染的出现。这是白唇西貒感染猪丹毒丝菌的首例报告,并且是对动物携带不同猪丹毒丝菌基因型的首次描述。